Ab087546

antibody from Aladdin Scientific Corporation

Targeting: ADIPOQ ACDC, ACRP30, adiponectin, apM1, GBP28

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

Ab087546

Provider

Aladdin Scientific Corporation

Product name

ADIPOQ Mouse mAb

Antibody type

Monoclonal

Description

Mouse monoclonal (H2) to ADIPOQ could be used for WB, IHC and so on.ApplicationWB: 0.29-2µg/mL;IHC: 5-20µg/mL;Protein FunctionImportant adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.Post-translationalHydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting. HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes. O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.

Reactivity

Human, Rat, Porcine

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

Ala108~Asn244


Antibody clone number

H2

Vial size

100μl,10μl,1ml,20μl,50μl

Concentration

Lot by Lot

Storage

Store at 4 ℃ for frequent use. Aliquot and store at -20 ℃ for 24 months. Upon delivery aliquot. Avoid freeze/thaw cycle.