Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [1]
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- Product number
- BS64008 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- TLK1 (phospho S743) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide derived from human TLK1 around the phosphorylation site of Serine 743.
- Description
- The Tousled-like kinases (TLK1 and TLK2, also designated PKU-ÉÇ and PKU-Ï«, respectively) are the human homologs of the Tousled gene from Arabidopsis thaliana, which encodes a Serine/Threonine kinase that is necessary for proper organ morphogenesis. Both TLKs contain a nuclear localization signal and a predicted coiled-coil region in the N-terminal domain. TLK is ubiquitously expressed, and is prevalent in mouse testis, especially in pachytene spermatocytes and round spermatids. It displays a propensity to dimerize through an interaction between its coiled-coil structure and is able to autophosphorylate, as well as phosphorylate exogenous substrates. TLK1 and TLK2 are regulated by the cell cycle, showing maximum activity during S phase. Subsequently, they are thought to regulate the development of multicellular organisms, including playing a key role in spermatogenesis, through a series of phosphorylations.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of p-TLK1 (S743) pAb at 1:500 dilutionLane1:MCF-7 whole cell lysateLane2:Rat brain tissue lysateLane3:H9C2 whole cell lysate