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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB3318 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3318, RRID:AB_1581365
- Product name
- TLK1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of TLK1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Suppression of Tousled-like kinase activity after DNA damage or replication block requires ATM, NBS1 and Chk1.
Mammalian homologues of the plant Tousled gene code for cell-cycle-regulated kinases with maximal activities linked to ongoing DNA replication.
Phosphorylation of SNAP-23 by the novel kinase SNAK regulates t-SNARE complex assembly.
Krause DR, Jonnalagadda JC, Gatei MH, Sillje HH, Zhou BB, Nigg EA, Khanna K
Oncogene 2003 Sep 4;22(38):5927-37
Oncogene 2003 Sep 4;22(38):5927-37
Mammalian homologues of the plant Tousled gene code for cell-cycle-regulated kinases with maximal activities linked to ongoing DNA replication.
Silljé HH, Takahashi K, Tanaka K, Van Houwe G, Nigg EA
The EMBO journal 1999 Oct 15;18(20):5691-702
The EMBO journal 1999 Oct 15;18(20):5691-702
Phosphorylation of SNAP-23 by the novel kinase SNAK regulates t-SNARE complex assembly.
Cabaniols JP, Ravichandran V, Roche PA
Molecular biology of the cell 1999 Dec;10(12):4033-41
Molecular biology of the cell 1999 Dec;10(12):4033-41
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of TLK1 polyclonal antibody (Cat # PAB3318) in HL-60 cell lysate. TLK1 (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with TLK1 polyclonal antibody (Cat # PAB3318) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
