Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PAB10238 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10238, RRID:AB_1715917
- Product name
- TLK1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of TLK1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references TLK1B is elevated with eIF4E overexpression in breast cancer.
Suppression of Tousled-like kinase activity after DNA damage or replication block requires ATM, NBS1 and Chk1.
Human Tousled like kinases are targeted by an ATM- and Chk1-dependent DNA damage checkpoint.
Norton KS, McClusky D, Sen S, Yu H, Meschonat C, Debenedetti A, Li BD
The Journal of surgical research 2004 Jan;116(1):98-103
The Journal of surgical research 2004 Jan;116(1):98-103
Suppression of Tousled-like kinase activity after DNA damage or replication block requires ATM, NBS1 and Chk1.
Krause DR, Jonnalagadda JC, Gatei MH, Sillje HH, Zhou BB, Nigg EA, Khanna K
Oncogene 2003 Sep 4;22(38):5927-37
Oncogene 2003 Sep 4;22(38):5927-37
Human Tousled like kinases are targeted by an ATM- and Chk1-dependent DNA damage checkpoint.
Groth A, Lukas J, Nigg EA, Silljé HH, Wernstedt C, Bartek J, Hansen K
The EMBO journal 2003 Apr 1;22(7):1676-87
The EMBO journal 2003 Apr 1;22(7):1676-87
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using TLK1 polyclonal antibody (Cat # PAB10238) shows detection of aband ~ 64 KDa corresponding to isoform 3 of human TLK1 (arrowhead).Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown).Approximately 30 ug of a HeLa nuclear extract was separated by SDS-PAGE and transferred onto nitrocellulose.After blocking the membrane was probed with the primary antibody diluted to 1 : 250 for 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-RabbitIgG [H&L] MX for 45 min at room temperature.