ABIN117936

antibody from antibodies-online

Targeting: TLK1 KIAA0137, PKU-BETA

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Antibody Data

Product number

ABIN117936

Provider

antibodies-online

Product name

anti-Tousled-Like Kinase 1 (TLK1) (Isoform 1), (AA 737-751), (pSer695) antibody

Antibody type

Polyclonal

Antigen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 737-751 of human Tlk1 (Tousled-like kinase) (isoform 1) protein.

Description

Immunoaffinity chromatography.

Reactivity

Human

Host

Rabbit

Antigen sequence

Human Tlk-1 (isoform 1), 766 aa, pr
edicted MW 86.7 kDa: 1 msvqsssgsl e
gppswsqls tsptpgsaaa arsllnhtpp sgr
pregamd elhsldprrq 61 ellearftgv as
gstgstgs csvgakastn nessnhsfgs lgsl
sdkese tpekkqsess 121 rgrkrkaenq ne
ssqgksig grghkisdyf eyqggngssp vrgi
ppairs pqnshshstp 181 sssvrpnsps pt
alafgdhp ivqpkqlsfk iiqtdltmlk laal
esnkiq dlekkegrid 241 dllrancdlr rq
ideqqkll ekykerlnkc ismskkllie kstq
eklssr eksmqdrlrl 301 ghfttvrhga sf
teqwtdgf afqnlvkqqe wvnqqredie rqrk
llakrk pptannsqap 361 stnsepkqrk nk
avngaend pfvrpnlpql ltlaeyheqe eifk
lrlghl kkeeaeiqae 421 lerlervrnl hi
relkrinn ednsqfkdhp tlnerylllh llgr
ggfsev ykafdlyeqr 481 yaavkihqln ks
wrdekken yhkhacreyr ihkeldhpri vkly
dyfsld tdtfctvley 541 cegndldfyl kq
hklmseke arsivmqivn alrylneikp piih
ydlkpg nillvdgtac 601 geikitdfgl sk
imdddsyg vdgmdltsqg agtywylppe cfvv
gkeppk isnkvdvwsv 661 gviffqclyg rk
pfghnqsq qdilqentil katevqfpvk pvvs
seakaf irrclayrke 721 drfdvhqlan dp
yllphmrr snssgnlhma gltasptpps ssii
ty


Epitope

Isoform 1,AA 737-751,pSer695

Vial size

0.1 mg

Concentration

0.6 mg/mL (by UV absorbance at 280 nm)

Storage

Store vial at -20° C or below prior to opening. For extended storage aliquot contents and freeze at -20°C or below.

Handling

Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

References

Submitted references

TLK1B is elevated with eIF4E overexpression in breast cancer.

Norton KS, McClusky D, Sen S, Yu H, Meschonat C, Debenedetti A, Li BD
The Journal of surgical research 2004 Jan;116(1):98-103

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Suppression of Tousled-like kinase activity after DNA damage or replication block requires ATM, NBS1 and Chk1.

Krause DR, Jonnalagadda JC, Gatei MH, Sillje HH, Zhou BB, Nigg EA, Khanna K
Oncogene 2003 Sep 4;22(38):5927-37

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Human Tousled like kinases are targeted by an ATM- and Chk1-dependent DNA damage checkpoint.

Groth A, Lukas J, Nigg EA, Silljé HH, Wernstedt C, Bartek J, Hansen K
The EMBO journal 2003 Apr 1;22(7):1676-87

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Mammalian homologues of the plant Tousled gene code for cell-cycle-regulated kinases with maximal activities linked to ongoing DNA replication.

Silljé HH, Takahashi K, Tanaka K, Van Houwe G, Nigg EA
The EMBO journal 1999 Oct 15;18(20):5691-702

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Phosphorylation of SNAP-23 by the novel kinase SNAK regulates t-SNARE complex assembly.

Cabaniols JP, Ravichandran V, Roche PA
Molecular biology of the cell 1999 Dec;10(12):4033-41

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cDNA cloning and chromosomal mapping of genes encoding novel protein kinases termed PKU-alpha and PKU-beta, which have nuclear localization signal.

Yamakawa A, Kameoka Y, Hashimoto K, Yoshitake Y, Nishikawa K, Tanihara K, Date T
Gene 1997 Nov 20;202(1-2):193-201

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[Amyloidosis of the gallbladder. An unusual localization].

Remy AJ, Perney P, Bourat L, Ramos J, Marty M, Cabaniols H, Durand L, Quenet F, Blanc F
Gastroenterologie clinique et biologique 1995 Feb;19(2):215-7

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Intracellular availability of unmodified, phosphorothioated and liposomally encapsulated oligodeoxynucleotides for antisense activity.

Thierry AR, Dritschilo A
Nucleic acids research 1992 Nov 11;20(21):5691-8