600-401-469

antibody from Rockland Immunochemicals, Inc.

Targeting: TLK1 KIAA0137, PKU-BETA

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-469

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-469, RRID:AB_2203875

Product name

Anti-Tlk1 (RABBIT) Antibody - 600-401-469

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's Affinity Purified anti-Tlk-1 antibody shows detection of a band ~64 kDa corresponding to isoform 3 of human Tlk-1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 30 ug of a HeLa nuclear extract was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:250 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results

Validation comment

Western Blot