R1497P

antibody from Acris Antibodies GmbH

Targeting: TLK1 KIAA0137, PKU-BETA

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

R1497P

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1497P, RRID:AB_1008028

Product name

anti TLK1 pSer695

Antibody type

Polyclonal

Antigen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 737-751 of human Tlk1 (Tousled-like kinase) (isoform 1) protein.

Reactivity

Human

Host

Rabbit

Vial size

0.1 mg

Concentration

0.6 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 1. Western blot using Affinity Purified anti-Tlk-1 antibody shows detection of aband ~64 kDa corresponding to isoform 3 of human Tlk-1 (arrowhead). Specific reactivity with this band is blocked when the antibody is preincubated with the immunizing peptide (data not shown). Approximately 30 µg of a HeLa nuclear extract was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:250 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye TM800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye TM800 fluorescence imagewas captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.