HPA016043

antibody from Atlas Antibodies

Targeting: TLK1 KIAA0137, PKU-BETA

Antibody data

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Validation data

Product number: HPA016043 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA016043, RRID:AB_1858027
Product name: Anti-TLK1
Antibody type: Polyclonal
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: ETPEKKQSESSRGRKRKAENQNESSQGKSIGGRGH KISDYFEYQGGNGSSPVRGIPPAIRSPQNSHSHST PSSSVRPNSPSPTALAFGDHPIVQPKQLSFKIIQT DLTMLKL
Isotype: IgG
Vial size: 100 µl
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

TLK1 protein structure - HPA016043 shown in red.

Enhanced validation

Submitted by: klas2
Enhanced method: Genetic validation
Main image
Experimental details: Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting TLK1 or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting TLK1 Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
Sample type: U-2 OS
Primary Ab dilution: 1:178
Conjugate: Horseradish Peroxidase
Secondary Ab: Secondary Ab
Secondary Ab dilution: 1:3000
Knockdown/Genetic Approaches Application: Western blot
KD Reagent Type: siRNA
Downregulation: >75%
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Prod no: s19122
KD Reagent Provider: Thermo Fisher Scientific
KD Reagent Prod no 2: s19123
KD Reagent Page 2: KD Reagent Page 2
KD Reagent Prod no 3: s19124
KD Reagent Page 3: KD Reagent Page 3
Antibody Lot Number: R06714
Appendix: 566d947ade076.pdf

Supportive validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TLK1 antibody. Remaining relative intensity is presented.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Recombinant expression validation
Main image
Experimental details: Western blot analysis in control (vector only transfected HEK293T lysate) and TLK1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402184).