HPA014757

antibody from Atlas Antibodies

Targeting: CYBRD1 CYB561A2, DCYTB, FLJ23462, FRRS3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA014757

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA014757, RRID:AB_1847374

Product name

Anti-CYBRD1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

VTRPQWKRPKEPNSTILHPNGGTEQGARGSMPAYS
GNNMDKSDSELNNEVAARKRNLALDEAGQRSTM


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines SK-MEL-30 and MCF-7 using Anti-CYBRD1 antibody. Corresponding CYBRD1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.

Sample type

HUMAN