PA5-120632

antibody from Invitrogen Antibodies

Targeting: GPR22

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-120632

Provider

Invitrogen Antibodies

Product name

GPR22 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive test controls include: U-87MG, U-251MG, SH-SY5Y, HepG2, Mouse heart, Mouse brain, Rat heart, Rat brain.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

6.34 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of GPR22 in various cell lines using a GPR22 Polyclonal antibody (Product # PA5-120632) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 90s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of GPR22 in L929 cells using a GPR22 Polyclonal antibody (Product # PA5-120632). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of GPR22 in L929 cells using a GPR22 Polyclonal antibody (Product # PA5-120632). Blue: DAPI for nuclear staining.