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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Flow cytometry [1]
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- Product number
- GTX62458 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX62458, RRID:AB_10620289
- Product name
- HES1 antibody [EPR4226]
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Protein A purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Storage
- Store at -20 °C. Stable for 12 months from date of receipt.
Submitted references Notch transactivates Rheb to maintain the multipotency of TSC-null cells.
Cho JH, Patel B, Bonala S, Manne S, Zhou Y, Vadrevu SK, Patel J, Peronaci M, Ghouse S, Henske EP, Roegiers F, Giannikou K, Kwiatkowski DJ, Mansouri H, Markiewski MM, White B, Karbowniczek M
Nature communications 2017 Nov 29;8(1):1848
Nature communications 2017 Nov 29;8(1):1848
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis on (A) fetal brain (B) PC-12 and (C) SH-SY5Y cell lysates using anti-HES1 RabMAb (cat. #GTX62458).
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of SH-SY5Y lysate (10 ?g) using HES1 antibody [EPR4226] at a dilution of 1:1000.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FACS analysis of SH-SY5Y cells fixed in 2% PFA using HES1 antibody [EPR4226] at a dilution of 1:30 (pink). Rabbit monoclonal IgG was used as an isotype control (green).
- Validation comment
- FACS
