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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- TA504001 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA504001, RRID:AB_11140744
- Product name
- HES1 mouse monoclonal antibody, clone OTI2D2 (formerly 2D2)
- Antibody type
- Monoclonal
- Description
- HES1 mouse monoclonal antibody, clone OTI2D2 (formerly 2D2)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- HES1
- Isotype
- IgG
- Antibody clone number
- OTI2D2
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HES1 (RC211709, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HES1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-HES1 monoclonal antibody.(1:200)
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-HES1 monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-HES1 mouse monoclonal antibody (TA504001) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HES1(RC211709).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HES1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504001)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-HES1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504001)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HES1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504001)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-HES1 antibody(TA504001),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
