PA5-42883

antibody from Invitrogen Antibodies

Targeting: ACSL3 ACS3, FACL3, PRO2194

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-42883

Provider

Invitrogen Antibodies

Product name

ACSL3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

TNF-α induces acyl-CoA synthetase 3 to promote lipid droplet formation in human endothelial cells.

Jung HS, Shimizu-Albergine M, Shen X, Kramer F, Shao D, Vivekanandan-Giri A, Pennathur S, Tian R, Kanter JE, Bornfeldt KE
Journal of lipid research 2020 Jan;61(1):33-44

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Metabolic enzyme ACSL3 is a prognostic biomarker and correlates with anticancer effectiveness of statins in non-small cell lung cancer.

Fernández LP, Merino M, Colmenarejo G, Moreno-Rubio J, Sánchez-Martínez R, Quijada-Freire A, Gómez de Cedrón M, Reglero G, Casado E, Sereno M, Ramírez de Molina A
Molecular oncology 2020 Dec;14(12):3135-3152

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Immunohistochemical staining reveals differential expression of ACSL3 and ACSL4 in hepatocellular carcinoma and hepatic gastrointestinal metastases.

Ndiaye H, Liu JY, Hall A, Minogue S, Morgan MY, Waugh MG
Bioscience reports 2020 Apr 30;40(4)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human 293T cell lysate using an anti-ACSL3 polyclonal antibody (Product # PA5-42883).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of THP-1 derived macrophage cells using an anti-ACSL3 polyclonal antibody (Product # PA5-42883).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of THP-1 derived macrophage cells using an anti-ACSL3 polyclonal antibody (Product # PA5-42883).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Immunohistochemistry reveals increased expression of both ACSL3 and ACSL4 in HCC Multiple liver tissues arrays were probed with antibodies specific for either ACSL3 or ACSL4. Representative examples (x20 maginfication) are shown for either ACSL3 or ACSL4 immunohistochemical staining of matched samples of HCC, normal liver, CCA and liver metastases.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Anti-ACSL3 IHC staining of HCC samples ACSL3 staining is present on lipid droplets (red arrows) and cytoplasmic reticular membranes (black arrow). Images were obtained at either x40 or x100 magnification.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Expression of ACSL3 and ACSL4 enzymes in hepatic malignancies Computer-aided, quantitative image analysis of ( A ) ACSL3 and ( B ) ACSL4 immunohistochemical staining of a liver tissue microarray for healthy controls, HCC, CCA and hepatic metastases (Met). Box-whisker plots with dot plots overlaid showing the median, interquartile range, minimum and maximum values, ACSL3 and ACSL4 staining values. Pairwise Wilcoxon rank sum tests were performed, significant P -values for which are shown on the graphs.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 Equilibrium distributions of ACSL3 and ACSL4 in sucrose density gradient fractions prepared from HepG2 cells Subcellular fractions isolated from HepG2 cells were separated by SDS/PAGE and Western blots were carried out to detect the lipid-droplet protein PNPLA3, ACSL3 and ACSL4, the ER marker protein calnexin, plasma membrane and lipid-raft associated flotillin, the Golgi protein GS28, the TGN-endosomal protein syntaxin-6, the early endosome-recruited protein EEA and the mitochondrial protein VDAC. Western blots are representative of experiments repeated three to four times.