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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-31227 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GTPBP9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, mouse testis, rat testis.
- Concentration
- 0.46 mg/mL
Submitted references Obg-Like ATPase 1 Enhances Chemoresistance of Breast Cancer via Activation of TGF-β/Smad Axis Cascades.
Liu J, Miao X, Xiao B, Huang J, Tao X, Zhang J, Zhao H, Pan Y, Wang H, Gao G, Xiao GG
Frontiers in pharmacology 2020;11:666
Frontiers in pharmacology 2020;11:666
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GTPBP9 using 30 µg of HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a GTPBP9 polyclonal antibody (Product # PA5-31227) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GTPBP9 using 50 µg of mouse brain lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a GTPBP9 polyclonal antibody (Product # PA5-31227) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GTPBP9 was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a GTPBP9 Polyclonal Antibody (Product # PA5-31227) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GTPBP9 was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a GTPBP9 Polyclonal Antibody (Product # PA5-31227) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GTPBP9 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a GTPBP9 Polyclonal Antibody (Product # PA5-31227) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GTPBP9 in methanol-fixed HeLa cells using a GTPBP9 polyclonal antibody (Product # PA5-31227) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using GTPBP9 (Product # PA5-31227) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Upregulated of OLA1 in acquired drug-resistant cell line MCF-7-PTR. (A) Morphology of paclitaxel-induced MCF-7-PTR cells and the parent MCF-7 cells (100X). (B) Drug resistance assay for enhanced expression of OLA1 promotes MCF-7-PTR cell resistance to PTX. (C) Drug resistance assay for enhanced expression of OLA1 promotes MCF-7-PTR cell resistance to 5-Fu. MCF-7 cells and MCF-7-PTR cells were analyzed for the presence of OLA1 by RT-PCR (D) , Western blotting (E) . The relative fold-change was compared with MCF-7 cells (* P < 0.05, ** P < 0.01, *** P < 0.001, Student's t-test).
