MA5-34771

antibody from Invitrogen Antibodies

Targeting: GOPC CAL, dJ94G16.2, FIG, GOPC1, PIST

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34771

Provider

Invitrogen Antibodies

Product name

PIST Recombinant Rabbit Monoclonal Antibody (JG79-35)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: SiHa, HepG2, MCF-7, SKOV-3, human liver cancer tissue, human colon tissue, human kidney tissue, mouse brain tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JG79-35

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PIST in SiHa cell. Samples were incubated with PIST monoclonal antibody (Product # MA5-34771), at a dilution of 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PIST in SKOV-3 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PIST monoclonal antibody (Product # MA5-34771), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PIST in MCF-7 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PIST monoclonal antibody (Product # MA5-34771), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PIST in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PIST monoclonal antibody (Product # MA5-34771), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PIST in paraffin-embedded mouse brain tissue. Samples were incubated with PIST monoclonal antibody (Product # MA5-34771), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PIST in paraffin-embedded human liver cancer tissue. Samples were incubated with PIST monoclonal antibody (Product # MA5-34771), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PIST in paraffin-embedded human kidney tissue. Samples were incubated with PIST monoclonal antibody (Product # MA5-34771), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PIST in paraffin-embedded human colon tissue. Samples were incubated with PIST monoclonal antibody (Product # MA5-34771), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of PIST in MCF-7 cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with PIST monoclonal antibody (Product # MA5-34771) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of PIST in MCF-7 cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with PIST monoclonal antibody (Product # MA5-34771) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.