12163-1-AP

antibody from Invitrogen Antibodies

Targeting: GOPC CAL, dJ94G16.2, FIG, GOPC1, PIST

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

12163-1-AP

Provider

Invitrogen Antibodies

Product name

GOPC Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MSAGGPCPA AAGGGPGGAS CSVGAPGGVS MFRWLEVLEK EFDKAFVDVD LLLGEIDPDQ ADITYEGRQK MTSLSSCFAQ LCHKAQSVSQ INHKLEAQLV DLKSELTETQ AEKVVLEKEV HDQLLQLHSI QLQLHAKTGQ SADSGTIKAK LERELEANKK EKMKEAQLEA EVKLLRKENE ALRRHIAVLQ AEVYGARLAA KYLDKELAGR VQQIQLLGRD MKGPAHDKLW NQLEAEIHLH RHKTVIRACR GRNDLKRPMQ APPGHDQDSL KKSQGVGPIR KVLLLKEDHE GLGISITGGK EHGVPILISE IHPGQPADRC GGLHVGDAIL AVNGVNLRDT KHKEAVTILS QQRGEIEFEV VYVAPEVDSD D (1-370 aa encoded by BC009553)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.27 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 12163-1-AP (GOPC antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed HEK-293T cells using GOPC antibody (Product # 12163-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HEK-293 cells using 12163-1-AP (GOPC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer using 12163-1-AP (GOPC antibody) at dilution of 1:50 (under 10x lens).