Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [6]
Submit
Validation data
Reference
Comment
Report error
- Product number
- OPA1-04020 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NMDAR2C Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- This antibody detects predominantly the NR2C isoform but may show low level cross-reactivity with the NR2A and NR2B isoforms. In Western blot, this antibody detects a ~140 kDa protein representing NR2C in rat brain homogenate. Faint bands representing the NR2A and NR2B isoforms at 180 KDa may also be observed. Immunohistochemical staining in rat brain with OPA1-04020 yields a pattern consistent with plasma membrane staining. Reconstitute with 50 µL of PBS.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 10 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Reconstitution of synaptic Ion channels from rodent and human brain in Xenopus oocytes: a biochemical and electrophysiological characterization.
Excitatory glycine responses of CNS myelin mediated by NR1/NR3 "NMDA" receptor subunits.
Glutamate signaling proteins and tyrosine hydroxylase in the locus coeruleus of alcoholics.
Elevated levels of the NR2C subunit of the NMDA receptor in the locus coeruleus in depression.
Mazzo F, Zwart R, Serratto GM, Gardinier KM, Porter W, Reel J, Maraula G, Sher E
Journal of neurochemistry 2016 Aug;138(3):384-96
Journal of neurochemistry 2016 Aug;138(3):384-96
Excitatory glycine responses of CNS myelin mediated by NR1/NR3 "NMDA" receptor subunits.
Piña-Crespo JC, Talantova M, Micu I, States B, Chen HS, Tu S, Nakanishi N, Tong G, Zhang D, Heinemann SF, Zamponi GW, Stys PK, Lipton SA
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Aug 25;30(34):11501-5
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Aug 25;30(34):11501-5
Glutamate signaling proteins and tyrosine hydroxylase in the locus coeruleus of alcoholics.
Karolewicz B, Johnson L, Szebeni K, Stockmeier CA, Ordway GA
Journal of psychiatric research 2008 Apr;42(5):348-55
Journal of psychiatric research 2008 Apr;42(5):348-55
Elevated levels of the NR2C subunit of the NMDA receptor in the locus coeruleus in depression.
Karolewicz B, Stockmeier CA, Ordway GA
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 2005 Aug;30(8):1557-67
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 2005 Aug;30(8):1557-67
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of NMDAR2C in rat cerebellar lysate (10 µg) using a NMDAR2C polyclonal antibody (Product # OPA1-04020), showing specific immunolabeling of a ~140 kDa band corresponding to the NR2C subunit of the NMDA receptor.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
