MA5-24246

antibody from Invitrogen Antibodies

Targeting: LAMA3 BM600-150kDa, epiligrin, kalinin-165kDa, LAMNA, nicein-150kDa

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24246

Provider

Invitrogen Antibodies

Product name

Laminin alpha-3 Monoclonal Antibody (546215)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, no cross reactivity with recombinant human Laminin alpha 1, beta 1, gamma 2, or recombinant mouse Laminin alpha 4 is observed.

Antibody clone number

546215

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Laminin alpha-3 in human lung tissue. Samples were incubated in Laminin alpha-3 monoclonal antibody (Product # MA5-24246) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for Laminin α3/Laminin‚5 at approximately 190 kDa (as indicated). This experiment was conducted under non-reducing conditions.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of Laminin alpha-3 in immersion fixed NHEK human normal epidermal keratinocytes treated with 10 ng/mL Recombinant Human TGF-beta 1 for 24 hours. Samples were incubated in Laminin alpha-3 monoclonal antibody (Product # MA5-24246) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to the perinuclear space.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Laminin alpha-3 in immersion fixed paraffin-embedded sections of human stomach. Samples were incubated with Laminin alpha-3 monoclonal antibody (Product # MA5-24246) using a dilution of 5 µg/mL for 1 hour at room temperature followed by Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic . Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of epithelial cells in gastric glands.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Laminin alpha-3 in U2OS human osteosarcoma cell line. Samples were incubated in Laminin alpha-3 monoclonal antibody (Product # MA5-24246) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer and permeabilized with Flow Cytometry Permeabilization/Wash Buffer.