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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- TA501311 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501311, RRID:AB_11124662
- Product name
- Anti-C17orf28 mouse monoclonal antibody, clone OTI2F4 (formerly 2F4)
- Antibody type
- Monoclonal
- Description
- Anti-C17orf28 mouse monoclonal antibody, clone OTI2F4 (formerly 2F4)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- HID1
- Isotype
- IgG
- Antibody clone number
- OTI2F4
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
Submitted references HID-1 is required for homotypic fusion of immature secretory granules during maturation.
Du W, Zhou M, Zhao W, Cheng D, Wang L, Lu J, Song E, Feng W, Xue Y, Xu P, Xu T
eLife 2016 Oct 18;5
eLife 2016 Oct 18;5
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C17orf28 (RC206740, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C17orf28.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-C17orf28 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-C17orf28 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-C17orf28 mouse monoclonal antibody (TA501311) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY C17orf28(RC206740).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-C17orf28 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501311, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-C17orf28 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501311, Dilution 1:50)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC206740 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-C17orf28 antibody(TA501311), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-C17orf28 antibody(TA501311),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
