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ImmunocytochemistryAntibody data
- Antibody Data
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- References [1]
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- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- NBP1-84563 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-84563, RRID:AB_11037450
- Product name
- Rabbit Polyclonal NT5C Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human NT5C antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Phosphoproteomic comparison of Pik3ca and Pten signalling identifies the nucleotidase NT5C as a novel AKT substrate.
Moniz LS, Surinova S, Ghazaly E, Velasco LG, Haider S, RodrÃguez-Prados JC, Berenjeno IM, Chelala C, Vanhaesebroeck B
Scientific reports 2017 Jan 6;7:39985
Scientific reports 2017 Jan 6;7:39985
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: NT5C Antibody [NBP1-84563] - Simple Western lane view shows a specific band for NT5C in 0.2 mg/ml of U-2OS lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: NT5C Antibody [NBP1-84563] - Simple Western lane view shows a specific band for NT5C in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: NT5C Antibody [NBP1-84563] - Electropherogram image(s) of corresponding Simple Western lane view. NT5C antibody was used at 1:20 dilution on U-2OS lysates(s).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NT5C Antibody [NBP1-84563] - Staining of human duodenum shows cytoplasmic positivity in glandular cells.
