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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-28462 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GGA3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: A431.
- Concentration
- 0.59 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GGA3 Polyclonal Antibody (Product # PA5-28462). Sample (30 µg of whole cell lysate). Lane A: A431 . 7.5% SDS PAGE. GGA3 Polyclonal Antibody (Product # PA5-28462) diluted at 1:2,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GGA3 Polyclonal Antibody detects GGA3 protein at Golgi apparatus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: GGA3 protein stained by GGA3 Polyclonal Antibody (Product # PA5-28462) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GGA3 Polyclonal Antibody detects GGA3 protein at cytoplasm on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. GGA3 Polyclonal Antibody (Product # PA5-28462) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using GGA3 (Product # PA5-28462) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
