Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A305-815A-T - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HNRNPA3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 10 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human HNRNPA3 by western blot. Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, Jurkat, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HNRNPA3 antibody (Product # A305-815A-T lot 1) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human HNRNPA3 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, HEK293T, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HNRNPA3 antibody A305-815A-M (lot A305-815A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human HNRNPA3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HNRNPA3 antibody (Product # A305-815A-T; lot 2) used for IP at 6 µg per reaction. HNRNPA3 was also immunoprecipitated by a previous lot of this antibody (Product # A305-815A-T; lot 1). For blotting immunoprecipitated HNRNPA3, Product # A305-815A-T was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
