20887-1-AP

antibody from Invitrogen Antibodies

Targeting: CALD1 CDM, H-CAD, L-CAD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

20887-1-AP

Provider

Invitrogen Antibodies

Product name

Caldesmon Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: EKPKRGSIG ENQIKDEKIK KDKEPKEEVK SFMDRKKGFT EVKSQNGEFM THKLKHTENT FSRPGGRASV DTKEAEGAPQ VEAGKRLEEL RRRRGETESE EFEKLKQKQQ EAALELEELK KKREERRKVL EEEEQRRKQE EADRKLREEE EKRRLKEEIE RRRAEAAEKR QKMPEDGLSD DKKPFKCFTP KGSSLKIEER AEFLNKSVQK SSGVKSTHQA AIVSKIDSRL EQYTSAIEGT KSAKPTKPAA SDLPVPAEGV RNIKSMWEKG NVFSSPTAAG TPNKETAGLK VGVSSRINEW LTKTPDGNKS PAPKPSDLRP GDVSSKRNLW EKQSVDKVTS PTKV (196-538 aa encoded by B C040354)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 20887-1-AP (Caldesmon antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 20887-1-AP (Caldesmon antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using CALD1 antibody 20887-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA ) fixed mouse heart tissue using 20887-1-AP (Caldesmon antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon tissue slide using 20887-1-AP (Caldesmon antibody at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon tissue slide using 20887-1-AP (Caldesmon antibody at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 20887-1-AP ( Caldesmon antibody at dilution of 1:100 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 20887-1-AP ( Caldesmon antibody at dilution of 1:100 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HeLa cells were intracellularly stained with 0.2 µg Anti-Human Caldesmon (Product # 20887-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (green), and 0.2 µg Control Antibody. Cells were fixed with 90% MeOH.