Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 677602 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#677602, RRID:AB_2565909
- Product name
- Purified anti-IL-11
- Antibody type
- Monoclonal
- Antigen
- Recombinant human IL-11, 180 amino acids Val20-Leu199 (Accession# P20809.1) was expressed in 293E.
- Description
- IL-11, a member of the IL-6 family of cytokines, exerts a wide range of biological effects on various cell types including hematopoietic cells, hepatocytes, adipocytes, neurons, and osteoblasts. IL-11 works synergistically with other growth factors including SCF, IL-4, IL-3, IL-7, IL-12, IL-13, and GM-CSF to stimulate the proliferation of cells from several hematopoietic lineages. Binding of IL-11 to IL-11 receptor (IL-11R) induces membrane bound gp130 homodimerization and triggers the STAT3 phosphorylation by JAK. IL-11 shares the common receptor subunit gp130 with IL-6, IL-27, LIF, OSM, CNTF, CT-1, CLC, and NP. Female mice deficient in IL-11R revealed that IL-11 has an important role in embryonic implantation. In addition, IL-11 shows anti-inflammatory activity in models of inflammatory bowel disease, chemotherapy induced oral mucositis, and inflammatory arthritis. IL-11 and IL-13 are highly expressed in asthmatic airways (Th2 response). IL-11 can inhibit Th1 responses and the production of Th1 cytokines such as IL-12, shifting inflammation in Th2 direction. Elevated IL-11 expression is associated with tumor grade and invasion in gastric cancer. Recombinant human IL-11 has been clinically approved to improve platelet recovery after chemotherapy-induced thrombocytopenia.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- M3103F11
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Recombinant human IL-11 (lane 1, 50 ng) and recombinant mouse IL-11 (lane 2, 50 ng) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-IL-11 antibody (clone M3103F11) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
- Conjugate
- Unconjugated