500-P01-1MG

antibody from Invitrogen Antibodies

Targeting: IL11 AGIF, IL-11

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

500-P01-1MG

Provider

Invitrogen Antibodies

Product name

IL-11 Polyclonal Antibody, PeproTech®

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

AA Sequence of recombinant protein: MPGPPPGPPR VSPDPRAELD STVLLTRSLL ADTRQLAAQL RDKFPADGDH NLDSLPTLAM SAGALGALQL PGVLTRLRAD LLSYLRHVQW LRRAGGSSLK TLEPELGTLQ ARLDRLLRRL QLLMSRLALP QPPPDPPAPP LAPPSSAWGG IRAAHAILGG LHLTLDWAVR GLLLLKTRL.

Concentration

0.1-1.0 mg/mL

References

Submitted references

LIM domain only 2 over-expression in prostate stromal cells facilitates prostate cancer progression through paracrine of Interleukin-11.

Jiang CY, Yu JJ, Ruan Y, Wang XH, Zhao W, Wang XJ, Zhu YP, Gao Y, Hao KY, Chen L, Han BM, Xia SJ, Zhao FJ
Oncotarget 2016 May 3;7(18):26247-58

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Interleukin-11 promotes the progress of gastric carcinoma via abnormally expressed versican.

Zhang Z, Zhang J, Miao L, Liu K, Yang S, Pan C, Jiao B
International journal of biological sciences 2012;8(3):383-93

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect Human IL-11 by Western Blot analysis IL-11 Polyclonal Antibody (Product # 500-P01-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-11 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect Human IL-11 by Western Blot analysis IL-11 Polyclonal Antibody (Product # 500-P01-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-11 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA: To detect Human IL-11 by sandwich ELISA (using 100 µL/well) a concentration of 0.5-2.0 µg/mL of IL-11 Polyclonal Antibody (Product # 500-P01-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech IL-11 Polyclonal Antibody, Biotin (Product # 500-P01BT-1MG) as a detection antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human IL-11.