NBP1-85574

antibody from Novus Biologicals

Targeting: CLIC4 CLIC4L, DKFZP566G223, H1, huH1, P64H1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-85574

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-85574, RRID:AB_11018841

Product name

Rabbit Polyclonal CLIC4 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human CLIC4 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Histamine H3 receptors aggravate cerebral ischaemic injury by histamine-independent mechanisms.

Yan H, Zhang X, Hu W, Ma J, Hou W, Zhang X, Wang X, Gao J, Shen Y, Lv J, Ohtsu H, Han F, Wang G, Chen Z
Nature communications 2014 Feb 25;5:3334

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Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.

Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E
Nature methods 2013 Apr;10(4):315-23

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Impact of genomic stability on protein expression in endometrioid endometrial cancer.

Lomnytska MI, Becker S, Gemoll T, Lundgren C, Habermann J, Olsson A, Bodin I, Engström U, Hellman U, Hellman K, Hellström AC, Andersson S, Mints M, Auer G
British journal of cancer 2012 Mar 27;106(7):1297-305

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CLIC4 Antibody [NBP1-85574] - Analysis in human cell lines SK-MEL-30 and MCF-7 using anti-CLIC4 antibody. Corresponding CLIC4 RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-85574] - Staining of human kidney shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-85574] - Staining of human skeletal muscle shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-85574] - Staining in human kidney and skeletal muscle tissues using anti-CLIC4 antibody. Corresponding CLIC4 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-85574] - Staining of human colon using Anti-CLIC4 antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-85574] - Human endometrium using Anti-CLIC4 antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-85574] - Staining of human colon, endometrium, kidney and skeletal muscle using Anti-CLIC4 antibody NBP1-85574 (A) shows similar protein distribution across tissues to independent antibody NBP2-49321 (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-85574] - Staining of human skeletal muscle using Anti-CLIC4 antibody NBP1-85574.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-85574] - Staining of human kidney using Anti-CLIC4 antibody NBP1-85574.