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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunohistochemistry [1]
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- Product number
- AF1130 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Cathepsin E Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects both pro and mature mouse Cathepsin E in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human Cathepsin E and less than 1% cross-reactivity with recombinant mouse Cathepsin D is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P70269- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Global Protease Activity Profiling Provides Differential Diagnosis of Pancreatic Cysts.
Gene targeting of the cysteine peptidase cathepsin H impairs lung surfactant in mice.
Ivry SL, Sharib JM, Dominguez DA, Roy N, Hatcher SE, Yip-Schneider MT, Schmidt CM, Brand RE, Park WG, Hebrok M, Kim GE, O'Donoghue AJ, Kirkwood KS, Craik CS
Clinical cancer research : an official journal of the American Association for Cancer Research 2017 Aug 15;23(16):4865-4874
Clinical cancer research : an official journal of the American Association for Cancer Research 2017 Aug 15;23(16):4865-4874
Gene targeting of the cysteine peptidase cathepsin H impairs lung surfactant in mice.
Bühling F, Kouadio M, Chwieralski CE, Kern U, Hohlfeld JM, Klemm N, Friedrichs N, Roth W, Deussing JM, Peters C, Reinheckel T
PloS one 2011;6(10):e26247
PloS one 2011;6(10):e26247
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cathepsin E in Mouse Lung. Cathepsin E was detected in perfusion fixed frozen sections of mouse lung using Goat Anti-Mouse Cathepsin E Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1130) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of type II alveolar cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
