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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-62904 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZNF580 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PRSSSPEAMD PPPPKAPPFP KAEGPSSTPS SAAGPRPPRL GRHLLIDANG
- Concentration
- 0.4 mg/mL
Submitted references Role and mechanism of the zinc finger protein ZNF580 in foam-cell formation.
Zhang Z, Qin X, Liu J, Li Y, Chen H, Xie H, Chen J, Li C, Tong Y, Yang M, Zhang M
Experimental and therapeutic medicine 2022 Sep;24(3):579
Experimental and therapeutic medicine 2022 Sep;24(3):579
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ZNF580 in Lane 1: Marker (kDa) 250, 130, 100, 70, 55, 35, 25, 15, 10; Lane 2: Human cell line HEK 293. Samples were probed using a ZNF580 Polyclonal Antibody (Product # PA5-62904).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ZNF580 in control (vector only transfected HEK293T lysate) and ZNF580 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a ZNF580 Polyclonal Antibody (Product # PA5-62904).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ZNF580 in human cell line U-2 OS shows positivity in nucleus. Samples were probed using a ZNF580 Polyclonal Antibody (Product # PA5-62904).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ZNF580 in human nasopharynx shows strong cytoplasmic and nuclear positivity in respiratory epithelial cells. Samples were probed using a ZNF580 Polyclonal Antibody (Product # PA5-62904).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 LV-ZNF580 and Si-ZNF580 lentivirus transfection. (A) Fluorescence microscopy was used to examine the transfection efficiency of LV-ZNF580 and Si-ZNF580 lentivirus (magnification, x200). Green fluorescence was visible in the cytoplasm and around the nucleus of transfected THP-1 cells. (B) The level of ZNF580 mRNA expression was measured by reverse transcription-quantitative PCR. (C) The levels of ZNF580 protein in THP-1 cells transfected with lentivirus were measured by western blot analysis. * P
