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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- ACR-060-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CRLR/CALCRL (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human THP-1 monocytic leukemia cell line lysate: - 1. Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060), (1:200). 2. Anti-CRLR/CALCRL (extracellular) Antibody , preincubated with CRLR/CALCRL (extracellular) Blocking Peptide (#BLP-CR060).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat dorsal root ganglion lysates (lanes 1 and 4), rat lung membranes (lanes 2 and 5) and rat heart membranes (lanes 3 and 6): - 1-3. Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060), (1:200). 4-6. Anti-CRLR/CALCRL (extracellular) Antibody , preincubated with CRLR/CALCRL (extracellular) Blocking Peptide (#BLP-CR060).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse heart lysates (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1, 2. Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060), (1:200). 3, 4. Anti-CRLR/CALCRL (extracellular) Antibody , preincubated with CRLR/CALCRL (extracellular) Blocking Peptide (#BLP-CR060).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human THP-1 monocytic leukemia cell line lysate: - 1. Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060), (1:200). 2. Anti-CRLR/CALCRL (extracellular) Antibody , preincubated with CRLR/CALCRL (extracellular) Blocking Peptide (#BLP-CR060).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of Calcitonin Receptor-Like Receptor in rat brain stem - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. CRLR staining (green) in the Raphe Magnus region is detected in neurons (arrows). B. Pre-incubation of the Antibody with CRLR/CALCRL (extracellular)Blocking Peptide (#BLP-CR060), suppresses staining. Cell nuclei are stained with DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of Calcitonin Receptor-Like Receptor in mouse brain stem - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-CRLR/CALCRL (extracellular) (extracellular) Antibody (#ACR-060), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. CRLR staining (green) in the Raphe Magnus region is detected in (green) in neurons (arrows). B. Pre-incubation of the Antibody with CRLR/CALCRL (extracellular)Blocking Peptide (#BLP-CR060), suppresses staining. Cell nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of CRLR in live intact human THP-1 monocytic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060), (2.5 µg) + goat- Anti-rabbit-FITC.
