12468-2-AP

antibody from Invitrogen Antibodies

Targeting: RECQL5 FLJ90603, RecQ5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

12468-2-AP

Provider

Invitrogen Antibodies

Product name

RECQL5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MSSHHTTFP FDPERRVRST LKKVFGFDSF KTPLQESATM AVVKGNKDVF VCMPTGAGKS LCYQLPALLA KGITIVVSPL IALIQDQVDH LLTLKVRVSS LNSKLSAQER KELLADLERE KPQTKILYIT PEMAASSSFQ PTLNSLVSRH LLSYLVVDEA HCVSQWGHDF RPDYLRLGAL RSRLGHAPCV ALTATATPQV QEDVFAALHL KKPVAIFKTP CFRANLFYDV QFKELISDPY GNLKDFCLKA LGQEADKGLS GCGIVYCRTR EACEQLAIEL SCRGVNAKAY HAGLKASERT LVQNDWMEEK VPVIVATISF GMGVDKANVR FVAHWNIAKS MAGYYQESGR A (1-350 aa encoded by BC016911)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 12468-2-AP (RECQL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 12468-2-AP (RECQL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 12468-2-AP (RECQL5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 12468-2-AP (RECQL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 12468-2-AP (RECQL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using RECQL5 antibody 12468-2-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IGG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer using 12468-2-AP (RECQL5 antibody) at dilution of 1:50 (under 10x lens).