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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP52184PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP52184PU-N, RRID:AB_11147920
- Product name
- anti IL12RB2 (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 759-788aa) of human IL12RB2.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- 0.5 mg/mL
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis in MDA-MB435 cell line lysates (35ug/lane) using IL12RB2 antibody Cat.-No. AP52184PU-N. This demonstrates the IL12RB2 antibody detected the IL12RB2 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in human tonsils tissue (formalin-fixed, paraffin-embedded) using IL12RB2 antibody Cat.-No. AP52184PU-N (C-term), followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL12RB2 antibody for IHC. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram) using IL12RB2 antibody Cat.-No. AP52184PU-N (C-term),  followed by FITC-conjugated goat-anti-rabbit secondary antibodies.
