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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [7]
- Other assay [1]
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- Product number
- PA5-53144 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PKP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VGNGNLHRTS SVPEYVYNLH LVENDFVGGR SPVPKTYDML KAGTTATYEG RWGRGTAQYS SQKSVEERSL RHPLRRLEIS PDSSPERAHY THSDYQYSQR SQAGHTLHHQ ESRRAALLVP PRY
- Concentration
- 0.08 mg/mL
Submitted references Proteomic characterization of GSK3β knockout shows altered cell adhesion and metabolic pathway utilisation in colorectal cancer cells.
Bowler-Barnett E, Martinez-Garcia FD, Sherwood M, Aleidan A, John S, Weston S, Wang Y, Divecha N, Skipp P, Ewing RM
PloS one 2021;16(11):e0246707
PloS one 2021;16(11):e0246707
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of PKP2 in human cell lines Caco-2 and SK-MEL-30 using a PKP2 Polyclonal Antibody (Product # PA5-53144). Corresponding PKP2 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PKP2 in human cell line A-431 shows positivity in cell junctions & nucleus but excluded from the nucleoli. Samples were probed using a PKP2 Polyclonal Antibody (Product # PA5-53144).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PKP2 in human kidney using PKP2 Polyclonal Antibody (Product # PA5-53144).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PKP2 in human testis using PKP2 Polyclonal Antibody (Product # PA5-53144).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PKP2 in human liver using PKP2 Polyclonal Antibody (Product # PA5-53144).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PKP2 in human heart muscle and skeletal muscle tissues using PKP2 Polyclonal Antibody (Product # PA5-53144). Corresponding PKP2 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PKP2 in human heart muscle, kidney, liver and testis using PKP2 Polyclonal Antibody (Product # PA5-53144) (A) shows similar protein distribution across tissues to an independent PKP2 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PKP2 in human heart muscle using PKP2 Polyclonal Antibody (Product # PA5-53144) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PKP2 in human skeletal muscle using PKP2 Polyclonal Antibody (Product # PA5-53144) shows low expression as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 10.1371/journal.pone.0246707.g003 Fig 3 A. Enriched pathways represented in differentially abundant proteins. Enriched pathways were identified using Pathway Studio from the set of differential proteins (p
