MA5-11971

antibody from Invitrogen Antibodies

Targeting: MYH11 SMHC, SMMHC

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-11971

Provider

Invitrogen Antibodies

Product name

MYH11 Monoclonal Antibody (SM-M10)

Antibody type

Monoclonal

Antigen

Other

Description

MA5-11971 targets Myosin Smooth Muscle Heavy Chain in IF and IHC (P) applications and shows reactivity with Bovine and Human samples.

Antibody clone number

SM-M10

Concentration

0.2 mg/mL

References

Submitted references

Characterization of Burn Eschar Pericytes.

Evdokiou A, Kanisicak O, Gierek S, Barry A, Ivey MJ, Zhang X, Bodnar RJ, Satish L
Journal of clinical medicine 2020 Feb 24;9(2)

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Differential and synergistic effects of mechanical stimulation and growth factor presentation on vascular wall function.

Liang MS, Koobatian M, Lei P, Swartz DD, Andreadis ST
Biomaterials 2013 Oct;34(30):7281-91

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Myosin Smooth Muscle Heavy Chain was performed using 70% confluent log phase C2C12 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MYH11 (SM-M10) Mouse Monoclonal Antibody (Product # MA5-11971) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human uterus stained with Myosin antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of smooth muscles.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Isolated cells are a heterogeneous population of pericytes. ( a ) Cultured pericytes from normal skin and burn eschar were analyzed for expression of the pericyte markers CD146 and NG2 along with the fibroblast marker S100A4. Representative images of the experiments performed on three different cultures derived from three different patients for normal skin pericytes, burn eschar pericytes and fibroblasts are shown. Images were captured using Eclipse 90i microscope and photographed with a DS-Qi1MC Digital Microscope. Scale bar: 100 muM. Quantitative analysis of ( b ) CD146, ( c ) NG2, and ( d ) S100A4 was performed. Quantitative analysis was performed in triplicate at 10x high powered fields using NIS-Elements AR3.1 software. Data represented as mean +- SEM of two independent studies. Statistical analysis was performed using Student's t test with p < 0.05 considered statistically significant. ** p < 0.01. The results show pericytes are greater than 90% positive for CD146 and NG2 and less than 15% positive for S100A4. The fibroblasts are greater than 90% positive for S100A4 and less than 10% positive for CD146 and NG2. ( e ) Shown is the representative images on the staining performed on normal skin pericytes and burn eschar pericytes derived from two different patients using SM22, myosin smooth muscle heavy chain (MHY)11, and Calponin-1 are shown. Scale bar: 30 uM.