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- Antibody Data
- Antigen structure
- References [11]
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NB110-60002 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-60002, RRID:AB_905358
- Product name
- Mouse Monoclonal Defensin alpha 5 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This is specific for HD5. It does not cross-react with HD6, lysozyme or sPLA2.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 2.35 mg/ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Alarin in different human intestinal epithelial cell types.
Alpha-defensins (α-Defs) in Crohn's disease: decrease of ileal α-Def 5 via permanent methylation and increase in plasma α-Def 1-3 concentrations offering biomarker utility.
LRRK2 but not ATG16L1 is associated with Paneth cell defect in Japanese Crohn's disease patients.
Differentiation of Human Pluripotent Stem Cells into Colonic Organoids via Transient Activation of BMP Signaling.
Paneth cell defects in Crohn's disease patients promote dysbiosis.
Profiling of differentially expressed proteins in stage IV colorectal cancers with good and poor outcomes.
Lowered expression of galectin-2 is associated with lymph node metastasis in gastric cancer.
[Identification of protein markers for serum diagnosis of cancer based on microRNA expression profiling].
Paneth cell antimicrobial peptides: topographical distribution and quantification in human gastrointestinal tissues.
Human defensin 5 expression in intestinal metaplasia of the upper gastrointestinal tract.
Reduced Paneth cell alpha-defensins in ileal Crohn's disease.
Jabari S, Schrödl F, Kaser-Eichberger A, Kofler B, Brehmer A
Histochemistry and cell biology 2019 Jun;151(6):513-520
Histochemistry and cell biology 2019 Jun;151(6):513-520
Alpha-defensins (α-Defs) in Crohn's disease: decrease of ileal α-Def 5 via permanent methylation and increase in plasma α-Def 1-3 concentrations offering biomarker utility.
Cerrillo E, Moret I, Iborra M, Ramos D, Busó E, Tortosa L, Sáez-González E, Nos P, Beltrán B
Clinical and experimental immunology 2018 Apr;192(1):120-128
Clinical and experimental immunology 2018 Apr;192(1):120-128
LRRK2 but not ATG16L1 is associated with Paneth cell defect in Japanese Crohn's disease patients.
Liu TC, Naito T, Liu Z, VanDussen KL, Haritunians T, Li D, Endo K, Kawai Y, Nagasaki M, Kinouchi Y, McGovern DP, Shimosegawa T, Kakuta Y, Stappenbeck TS
JCI insight 2017 Mar 23;2(6):e91917
JCI insight 2017 Mar 23;2(6):e91917
Differentiation of Human Pluripotent Stem Cells into Colonic Organoids via Transient Activation of BMP Signaling.
Múnera JO, Sundaram N, Rankin SA, Hill D, Watson C, Mahe M, Vallance JE, Shroyer NF, Sinagoga KL, Zarzoso-Lacoste A, Hudson JR, Howell JC, Chatuvedi P, Spence JR, Shannon JM, Zorn AM, Helmrath MA, Wells JM
Cell stem cell 2017 Jul 6;21(1):51-64.e6
Cell stem cell 2017 Jul 6;21(1):51-64.e6
Paneth cell defects in Crohn's disease patients promote dysbiosis.
Liu TC, Gurram B, Baldridge MT, Head R, Lam V, Luo C, Cao Y, Simpson P, Hayward M, Holtz ML, Bousounis P, Noe J, Lerner D, Cabrera J, Biank V, Stephens M, Huttenhower C, McGovern DP, Xavier RJ, Stappenbeck TS, Salzman NH
JCI insight 2016 Jun 2;1(8):e86907
JCI insight 2016 Jun 2;1(8):e86907
Profiling of differentially expressed proteins in stage IV colorectal cancers with good and poor outcomes.
Kim HJ, Kang UB, Lee H, Jung JH, Lee ST, Yu MH, Kim H, Lee C
Journal of proteomics 2012 Jun 6;75(10):2983-97
Journal of proteomics 2012 Jun 6;75(10):2983-97
Lowered expression of galectin-2 is associated with lymph node metastasis in gastric cancer.
Jung JH, Kim HJ, Yeom J, Yoo C, Shin J, Yoo J, Kang CS, Lee C
Journal of gastroenterology 2012 Jan;47(1):37-48
Journal of gastroenterology 2012 Jan;47(1):37-48
[Identification of protein markers for serum diagnosis of cancer based on microRNA expression profiling].
Bukurova IuA, Nikitina SL, Khankin SL, Krasnov GS, Lisitsin NA, Karpov VL, Beresten' SF
Molekuliarnaia biologiia 2011 Mar-Apr;45(2):376-81
Molekuliarnaia biologiia 2011 Mar-Apr;45(2):376-81
Paneth cell antimicrobial peptides: topographical distribution and quantification in human gastrointestinal tissues.
Wehkamp J, Chu H, Shen B, Feathers RW, Kays RJ, Lee SK, Bevins CL
FEBS letters 2006 Oct 2;580(22):5344-50
FEBS letters 2006 Oct 2;580(22):5344-50
Human defensin 5 expression in intestinal metaplasia of the upper gastrointestinal tract.
Shen B, Porter EM, Reynoso E, Shen C, Ghosh D, Connor JT, Drazba J, Rho HK, Gramlich TL, Li R, Ormsby AH, Sy MS, Ganz T, Bevins CL
Journal of clinical pathology 2005 Jul;58(7):687-94
Journal of clinical pathology 2005 Jul;58(7):687-94
Reduced Paneth cell alpha-defensins in ileal Crohn's disease.
Wehkamp J, Salzman NH, Porter E, Nuding S, Weichenthal M, Petras RE, Shen B, Schaeffeler E, Schwab M, Linzmeier R, Feathers RW, Chu H, Lima H Jr, Fellermann K, Ganz T, Stange EF, Bevins CL
Proceedings of the National Academy of Sciences of the United States of America 2005 Dec 13;102(50):18129-34
Proceedings of the National Academy of Sciences of the United States of America 2005 Dec 13;102(50):18129-34
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Defensin alpha 5 Antibody (8C8) [NB110-60002] - Human skeletal muscle myocytes showing moderate nuclear and faint cytoplasmic staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Defensin alpha 5 Antibody (8C8) [NB110-60002] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using Defensin alpha 5 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
