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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- AF5536 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal Sphingosine Kinase 1/SPHK1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human SPHK1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human SPHK2 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Pseudomonas-derived ceramidase induces production of inflammatory mediators from human keratinocytes via sphingosine-1-phosphate.
Oizumi A, Nakayama H, Okino N, Iwahara C, Kina K, Matsumoto R, Ogawa H, Takamori K, Ito M, Suga Y, Iwabuchi K
PloS one 2014;9(2):e89402
PloS one 2014;9(2):e89402
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Sphingosine Kinase 1/SPHK1 by Western Blot. Detection of Human Sphingosine Kinase 1/SPHK1 by Western Blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Human Sphingosine Kinase 1/SPHK1 ELISA Standard Curve. Human Sphingosine Kinase 1/SPHK1 ELISA Standard Curve.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sphingosine Kinase 1/SPHK1 in Human Prostate. Sphingosine Kinase 1/SPHK1 in Human Prostate.
