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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- TA507215 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA507215, RRID:AB_2624142
- Product name
- SPHK1 mouse monoclonal antibody, clone OTI1A6 (formerly 1A6)
- Antibody type
- Monoclonal
- Description
- SPHK1 mouse monoclonal antibody, clone OTI1A6 (formerly 1A6)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SPHK1
- Isotype
- IgG
- Antibody clone number
- OTI1A6
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPHK1 (RC203398, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPHK1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SPHK1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-SPHK1 mouse monoclonal antibody (TA507215) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPHK1(RC203398).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-SPHK1 mouse monoclonal antibody (TA507215).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SPHK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507215)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-SPHK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507215)
- Validation comment
- IHC
