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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP13922PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP13922PU-N, RRID:AB_1771252
- Product name
- anti Sphingosine kinase 1 (SPHK1) (Center)
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the central region of Human SPHK1.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- 2.0 mg/mL
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- The anti-SPHK1 Pab (AP13922PU-N) is used in Western blot to detect SPHK1 in mouse kidney tissue lysate.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of anti-SPHK1 Antibody (Center) (AP13922PU-N) in 293 cell line lysates (35 µg/lane). SPHK1 (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue (hepatocarcinoma) reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
