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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA324639 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal ATG16L Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal ATG16L Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ATG16L1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- APG16L Antibody (Cat. #TA324639) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the APG16L antibody detected the APG16L protein (arrow).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Cos7, HEK293, MEF, and Hela cells, left to right, respectively. Data courtesy of Drs. Jiefei Geng and Dan Klionsky, University of Michigan.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of U251 cells, using ATG16L Antibody(Cat. #TA324639). U251 cells(right) were treated with Chloroquine (50 ??M,16h). TA324639 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with Autophagy APG16L antibody (L176), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
