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LearnNB110-60928
antibody from Novus Biologicals
Targeting: ATG16L1
APG16L, ATG16A, ATG16L, FLJ10035, WDR30
Western blot
Immunoelectron microscopyAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NB110-60928 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-60928, RRID:AB_925263
- Product name
- Rabbit Polyclonal ATG16L1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.65 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Detection and analysis of autophagy in the American alligator (Alligator mississippiensis).
MicroRNA-20a regulates autophagy related protein-ATG16L1 in hypoxia-induced osteoclast differentiation.
Overexpression of autophagy-related 16-like 1 in patients with oral squamous cell carcinoma.
Identification of autophagic vacuoles and regulators of autophagy in villous trophoblast from normal term pregnancies and in fetal growth restriction.
Amino acid starvation induced by invasive bacterial pathogens triggers an innate host defense program.
NOD2 enhances the innate response of alveolar macrophages to Mycobacterium tuberculosis in humans.
Crohn's disease-associated ATG16L1 polymorphism modulates pro-inflammatory cytokine responses selectively upon activation of NOD2.
Hale A, Merchant M, White M
Journal of experimental zoology. Part B, Molecular and developmental evolution 2020 May;334(3):192-207
Journal of experimental zoology. Part B, Molecular and developmental evolution 2020 May;334(3):192-207
MicroRNA-20a regulates autophagy related protein-ATG16L1 in hypoxia-induced osteoclast differentiation.
Sun KT, Chen MY, Tu MG, Wang IK, Chang SS, Li CY
Bone 2015 Apr;73:145-53
Bone 2015 Apr;73:145-53
Overexpression of autophagy-related 16-like 1 in patients with oral squamous cell carcinoma.
Tang JY, Hsi E, Huang YC, Hsu NC, Yang WC, Chang HW, Chai CY, Chu PY
Pathology oncology research : POR 2015 Apr;21(2):301-5
Pathology oncology research : POR 2015 Apr;21(2):301-5
Identification of autophagic vacuoles and regulators of autophagy in villous trophoblast from normal term pregnancies and in fetal growth restriction.
Curtis S, Jones CJ, Garrod A, Hulme CH, Heazell AE
The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians 2013 Mar;26(4):339-46
The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians 2013 Mar;26(4):339-46
Amino acid starvation induced by invasive bacterial pathogens triggers an innate host defense program.
Tattoli I, Sorbara MT, Vuckovic D, Ling A, Soares F, Carneiro LA, Yang C, Emili A, Philpott DJ, Girardin SE
Cell host & microbe 2012 Jun 14;11(6):563-75
Cell host & microbe 2012 Jun 14;11(6):563-75
NOD2 enhances the innate response of alveolar macrophages to Mycobacterium tuberculosis in humans.
Juárez E, Carranza C, Hernández-Sánchez F, León-Contreras JC, Hernández-Pando R, Escobedo D, Torres M, Sada E
European journal of immunology 2012 Apr;42(4):880-9
European journal of immunology 2012 Apr;42(4):880-9
Crohn's disease-associated ATG16L1 polymorphism modulates pro-inflammatory cytokine responses selectively upon activation of NOD2.
Plantinga TS, Crisan TO, Oosting M, van de Veerdonk FL, de Jong DJ, Philpott DJ, van der Meer JW, Girardin SE, Joosten LA, Netea MG
Gut 2011 Sep;60(9):1229-35
Gut 2011 Sep;60(9):1229-35
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATG16L1 Antibody [NB110-60928] - Detection of ATG16L1 using NB110-60928 in HeLa whole cell extracts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATG16L1 Antibody [NB110-60928] - Detection of ATG16L1 using NB110-60928 in HCT116 whole cell extracts. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ATG16L1 Antibody [NB110-60928] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using ATG16L1 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of ATG16L1 was observed. Staining was performed by Histowiz.
