Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 11-10020-25 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-APG16L Antibody
- Antibody type
- Polyclonal
- Description
- ATG16L1/APG16L is part of a large protein complex that functions as a molecular scaffold mediating protein-protein interactions necessary for formation of the autophagosome in response to both classical and pathogen-related autophagy stimuli. APG16L protein contains an N-terminal Atg5-binding domain, a coiled-coil domain and a C-terminal WD (tryptophan-aspartic acid)-repeat domain. APG16L forms a complex with the Atg12 Atg5 conjugate and together they are actively translocated to the phagophore and are further elongated during autophagosome formation. Genetic variation in the APG16L gene has been recently implicated in Crohn's disease pathogenesis. Polymorphism within APG16L gene results in excessive production of IL-1beta and IL-6 culmunating in the inflammatory process in Crohn's disease.
- Reactivity
- Human
- Host
- Rabbit
- Antigen sequence
A partial length recombinant APG16L
protein (amino acids 35-260) was u
sed as the immunogen for this antib
ody.- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freezing and thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
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- Experimental details
- Western blot analysis of APG16L. Anti-APG16L antibody (11-10020) was used at 4 µg/ml on Spleen lysate.