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LearnOAAB06654
antibody from Aviva Systems Biology
Targeting: MAP1LC3A
ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- OAAB06654 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- LC3 antibody
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 166AT1234
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of anti-LC3 Mab (Cat. #OAAB06654) at 8µg/ml. Lane 1: Y79 (soluble fraction of cell extract); Lane 2: 293 transfected with human LC3 (whole cell extract).
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- 10X( lower panel) and 20X (upper panel) immunohistochemistry images from muscle tissue of a diseased mouse off Dox after 5 weeks on regular food. Several fibers that have autophagic vesicles throughout are visible. Primary antibody used is Cat# OAAB06654. Data courtesy of Dr. Christy Caudill, Cincinnati Children's Hospital Medical Center.
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NCI-H460 cells using SOX2 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
