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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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- Product number
- TA324327 - Provider product page
- Provider
- OriGene
- Product name
- Mouse monoclonal LC3 Antibody (APG8)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal LC3 Antibody (APG8)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- MAP1LC3A
- Isotype
- IgG
- Antibody clone number
- 166AT1234
- Vial size
- 400 µl
- Concentration
- 1.09 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of anti-LC3 Mab (Cat. #TA324327) at 8 ug/ml. Lane 1: Y79 (soluble fraction of cell extract); Lane 2: 293 transfected with human LC3 (whole cell extract).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of anti-LC3 Mab (Cat. #TA324327) Hela cell lysates, which were treated with rapamycin or bafilomycin overnight. Data courtesy of Dr. David Rubinsztein, Cambridge Institute for Medical Research.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8)(Cat. #TA324327). U251 cells(right) were treated with Chloroquine (50 ??M,16h). TA324327 was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8) (Cat. #TA324327). U251 cells(right) were treated with Chloroquine (50 ??M,16h). TA324327 was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of U251 cells stained with TA324327 LC3 (APG8) antibody.U251 cells were treated with Chloroquine , then incubated with TA324327 LC3 (APG8) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- 10X( lower panel) and 20X (upper panel) immunohistochemistry images from muscle tissue of a diseased mouse off Dox after 5 weeks on regular food. Several fibers that have autophagic vesicles throughout are visible. Primary antibody used is Cat# TA324327. Data courtesy of Dr. Christy Caudill, Cincinnati Children's Hospital Medical Center.
- Validation comment
- IHC
