TA336735

antibody from OriGene

Targeting: MAP1LC3A ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA336735

Provider

OriGene

Product name

Rabbit Polyclonal LC3/MAP1LC3A Antibody

Antibody type

Polyclonal

Description

Rabbit Polyclonal LC3/MAP1LC3A Antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

MAP1LC3A

Antibody clone number

NULL

Vial size

100 µl

Concentration

0.2 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot: LC3 Antibody [TA336735] - Human brain lysate.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: LC3 Antibody [TA336735] - LC3 antibody was tested in HeLa cells with Dylight 488 (green). Cells were treated overnight with 50uM chloroquine to induce autophagosome formation. Nuclei and alpha-tubulin were counterst

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry: LC3 Antibody [TA336735] - Staining of LC3 in mouse liver.

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow Cytometry: LC3 Antibody [TA336735] - Staining of NTERA-2 cells using TA336735 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit IgG secondary antibody.

Validation comment

FC