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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [13]
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- Validations
- Western blot [4]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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- Product number
- GTX17380 - Provider product page
- Provider
- GeneTex
- Product name
- LC3A antibody [166AT1234]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Storage
- Maintain refrigerated at 2-8¢XC for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Chronic pulmonary exposure to traffic-related fine particulate matter causes brain impairment in adult rats.
Male meiotic cytokinesis requires ceramide synthase 3-dependent sphingolipids with unique membrane anchors.
GMI, an Immunomodulatory Protein from Ganoderma microsporum, Potentiates Cisplatin-Induced Apoptosis via Autophagy in Lung Cancer Cells.
CD40 ligand exhibits a direct antiviral effect on Herpes Simplex Virus type-1 infection via a PI3K-dependent, autophagy-independent mechanism.
Atorvastatin induces autophagic cell death in prostate cancer cells in vitro.
The effect of RNAi silencing of p62 using an osmotic polysorbitol transporter on autophagy and tumorigenesis in lungs of K-rasLA1 mice.
Induction of autophagy is essential for monocyte-macrophage differentiation.
Beclin 1 knockdown inhibits autophagic activation and prevents the secondary neurodegenerative damage in the ipsilateral thalamus following focal cerebral infarction.
Production of interferon α by human immunodeficiency virus type 1 in human plasmacytoid dendritic cells is dependent on induction of autophagy.
Mouse knock-out of IOP1 protein reveals its essential role in mammalian cytosolic iron-sulfur protein biogenesis.
The unfolded protein response protects human tumor cells during hypoxia through regulation of the autophagy genes MAP1LC3B and ATG5.
Characterization of Puma-dependent and Puma-independent neuronal cell death pathways following prolonged proteasomal inhibition.
The Rac1/MKK7/JNK pathway signals upregulation of Atg5 and subsequent autophagic cell death in response to oncogenic Ras.
Shih CH, Chen JK, Kuo LW, Cho KH, Hsiao TC, Lin ZW, Lin YS, Kang JH, Lo YC, Chuang KJ, Cheng TJ, Chuang HC
Particle and fibre toxicology 2018 Nov 9;15(1):44
Particle and fibre toxicology 2018 Nov 9;15(1):44
Male meiotic cytokinesis requires ceramide synthase 3-dependent sphingolipids with unique membrane anchors.
Rabionet M, Bayerle A, Jennemann R, Heid H, Fuchser J, Marsching C, Porubsky S, Bolenz C, Guillou F, Gröne HJ, Gorgas K, Sandhoff R
Human molecular genetics 2015 Sep 1;24(17):4792-808
Human molecular genetics 2015 Sep 1;24(17):4792-808
GMI, an Immunomodulatory Protein from Ganoderma microsporum, Potentiates Cisplatin-Induced Apoptosis via Autophagy in Lung Cancer Cells.
Hsin IL, Ou CC, Wu MF, Jan MS, Hsiao YM, Lin CH, Ko JL
Molecular pharmaceutics 2015 May 4;12(5):1534-43
Molecular pharmaceutics 2015 May 4;12(5):1534-43
CD40 ligand exhibits a direct antiviral effect on Herpes Simplex Virus type-1 infection via a PI3K-dependent, autophagy-independent mechanism.
Vlahava VM, Eliopoulos AG, Sourvinos G
Cellular signalling 2015 Jun;27(6):1253-63
Cellular signalling 2015 Jun;27(6):1253-63
Atorvastatin induces autophagic cell death in prostate cancer cells in vitro.
He Z, Yuan J, Qi P, Zhang L, Wang Z
Molecular medicine reports 2015 Jun;11(6):4403-8
Molecular medicine reports 2015 Jun;11(6):4403-8
The effect of RNAi silencing of p62 using an osmotic polysorbitol transporter on autophagy and tumorigenesis in lungs of K-rasLA1 mice.
Islam MA, Shin JY, Yun CH, Cho CS, Seo HW, Chae C, Cho MH
Biomaterials 2014 Feb;35(5):1584-96
Biomaterials 2014 Feb;35(5):1584-96
Induction of autophagy is essential for monocyte-macrophage differentiation.
Zhang Y, Morgan MJ, Chen K, Choksi S, Liu ZG
Blood 2012 Mar 22;119(12):2895-905
Blood 2012 Mar 22;119(12):2895-905
Beclin 1 knockdown inhibits autophagic activation and prevents the secondary neurodegenerative damage in the ipsilateral thalamus following focal cerebral infarction.
Xing S, Zhang Y, Li J, Zhang J, Li Y, Dang C, Li C, Fan Y, Yu J, Pei Z, Zeng J
Autophagy 2012 Jan;8(1):63-76
Autophagy 2012 Jan;8(1):63-76
Production of interferon α by human immunodeficiency virus type 1 in human plasmacytoid dendritic cells is dependent on induction of autophagy.
Zhou D, Kang KH, Spector SA
The Journal of infectious diseases 2012 Apr 15;205(8):1258-67
The Journal of infectious diseases 2012 Apr 15;205(8):1258-67
Mouse knock-out of IOP1 protein reveals its essential role in mammalian cytosolic iron-sulfur protein biogenesis.
Song D, Lee FS
The Journal of biological chemistry 2011 May 6;286(18):15797-805
The Journal of biological chemistry 2011 May 6;286(18):15797-805
The unfolded protein response protects human tumor cells during hypoxia through regulation of the autophagy genes MAP1LC3B and ATG5.
Rouschop KM, van den Beucken T, Dubois L, Niessen H, Bussink J, Savelkouls K, Keulers T, Mujcic H, Landuyt W, Voncken JW, Lambin P, van der Kogel AJ, Koritzinsky M, Wouters BG
The Journal of clinical investigation 2010 Jan;120(1):127-41
The Journal of clinical investigation 2010 Jan;120(1):127-41
Characterization of Puma-dependent and Puma-independent neuronal cell death pathways following prolonged proteasomal inhibition.
Tuffy LP, Concannon CG, D'Orsi B, King MA, Woods I, Huber HJ, Ward MW, Prehn JH
Molecular and cellular biology 2010 Dec;30(23):5484-501
Molecular and cellular biology 2010 Dec;30(23):5484-501
The Rac1/MKK7/JNK pathway signals upregulation of Atg5 and subsequent autophagic cell death in response to oncogenic Ras.
Byun JY, Yoon CH, An S, Park IC, Kang CM, Kim MJ, Lee SJ
Carcinogenesis 2009 Nov;30(11):1880-8
Carcinogenesis 2009 Nov;30(11):1880-8
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-MAP1LC3A Antibody at 1:5000 dilution + recombinant protein Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjμgated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- All lanes : Anti-MAP1LC3A Antibody at 1:2000 dilution Lane 1: human brain lysates Lane 2: mouse brain lysates Lane 3: rat brain lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjμgated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of anti-LC3 Mab at 8 μg/ml. Lane 1: Y79 (soluble fraction of cell extract); Lane 2: 293 transfected with human LC3 (whole cell extract).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of anti-LC3 Mab Hela cell lysates, which were treated with rapamycin or bafilomycin overnight. Data courtesy of Dr. David Rubinsztein, Cambridge Institute for Medical Research.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8). U251 cells(right) were treated with Chloroquine (50 £gM,16h). GTX17380 was diluted at 1:25 dilution. Dylight Fluor 488-conjμgated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8). U251 cells(right) were treated with Chloroquine (50 £gM,16h). GTX17380 was diluted at 1:25 dilution. Dylight Fluor 488-conjμgated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Fluorescent image of U251 cells stained with GTX17380 LC3 (APG8) antibody.U251 cells were treated with Chloroquine (50 £gM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with GTX17380 LC3 (APG8) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa FluorR 488 conjμgated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- 10X( lower panel) and 20X (upper panel) immunohistochemistry images from muscle tissue of a diseased mouse off Dox after 5 weeks on regular food. Several fibers that have autophagic vesicles throμghout are visible. Primary antibody used is LC3 Antibody. Data courtesy of Dr. Christy Caudill, Cincinnati Children's Hospital Medical Center.
