PAB29558

antibody from Abnova Corporation

Targeting: MAP1LC3A ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29558

Provider

Abnova Corporation

Product name

MAP1LC3A polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against partial synthetic protein of human MAP1LC3A.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Rat brain tissue lysates, Lane 2: Rat brain tissue lysates treated with blocking peptide with MAP1LC3A polyclonal antibody (Cat # PAB29558) at 1:1000 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of Chloroquine (50 uM, 16h) treated U251 cells with MAP1LC3A polyclonal antibody (Cat # PAB29558) shows positivity in cytoplasmic autophagic vacuoles at 1:200 dilution.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human brain tissue with MAP1LC3A polyclonal antibody (Cat # PAB29558) at 1:50-100 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)