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- Antibody Data
- Antigen structure
- References [18]
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- Western blot [3]
- Immunohistochemistry [2]
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- Product number
- NBP1-19167 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-19167, RRID:AB_1642513
- Product name
- Rabbit Polyclonal LC3A Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Although specificity between LC3A and LC3B has not been tested, this antibody was created to a peptide that has 100% identity to LC3A and 62% identity to LC3B.
- Reactivity
- Human, Mouse, Rat, Bovine, Zebrafish
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Hypoxia Prevents Mitochondrial Dysfunction and Senescence in Human c-Kit+ Cardiac Progenitor Cells.
Autophagy Sustains Pancreatic Cancer Growth through Both Cell-Autonomous and Nonautonomous Mechanisms.
Autophagic vacuoles in cardiomyocytes of dilated cardiomyopathy with initially decompensated heart failure predict improved prognosis.
Effector Protein Cig2 Decreases Host Tolerance of Infection by Directing Constitutive Fusion of Autophagosomes with the Coxiella-Containing Vacuole.
Rheb promotes cancer cell survival through p27Kip1-dependent activation of autophagy.
A dual role for autophagy in a murine model of lung cancer.
Phospholipase D2 mediates survival signaling through direct regulation of Akt in glioblastoma cells.
Autophagy is critical for pancreatic tumor growth and progression in tumors with p53 alterations.
mTORC1 inhibition delays growth of neurofibromatosis type 2 schwannoma.
Augmenting autophagy to treat acute kidney injury during endotoxemia in mice.
Pazopanib and sunitinib trigger autophagic and non-autophagic death of bladder tumour cells.
Macroautophagy abnormality in essential tremor.
Autophagy induced by resveratrol prevents human prion protein-mediated neurotoxicity.
Involvement of macroautophagy in multiple system atrophy and protein aggregate formation in oligodendrocytes.
Melatonin attenuates kainic acid-induced neurotoxicity in mouse hippocampus via inhibition of autophagy and α-synuclein aggregation.
"Autophagic flux" in normal mouse tissues: focus on endogenous LC3A processing.
Silica nanoconstruct cellular toleration threshold in vitro.
Pathogenic lysosomal depletion in Parkinson's disease.
Korski KI, Kubli DA, Wang BJ, Khalafalla FG, Monsanto MM, Firouzi F, Echeagaray OH, Kim T, Adamson RM, Dembitsky WP, Gustafsson ÅB, Sussman MA
Stem cells (Dayton, Ohio) 2019 Apr;37(4):555-567
Stem cells (Dayton, Ohio) 2019 Apr;37(4):555-567
Autophagy Sustains Pancreatic Cancer Growth through Both Cell-Autonomous and Nonautonomous Mechanisms.
Yang A, Herter-Sprie G, Zhang H, Lin EY, Biancur D, Wang X, Deng J, Hai J, Yang S, Wong KK, Kimmelman AC
Cancer discovery 2018 Mar;8(3):276-287
Cancer discovery 2018 Mar;8(3):276-287
Autophagic vacuoles in cardiomyocytes of dilated cardiomyopathy with initially decompensated heart failure predict improved prognosis.
Saito T, Asai K, Sato S, Hayashi M, Adachi A, Sasaki Y, Takano H, Mizuno K, Shimizu W
Autophagy 2016;12(3):579-87
Autophagy 2016;12(3):579-87
Effector Protein Cig2 Decreases Host Tolerance of Infection by Directing Constitutive Fusion of Autophagosomes with the Coxiella-Containing Vacuole.
Kohler LJ, Reed ShC, Sarraf SA, Arteaga DD, Newton HJ, Roy CR
mBio 2016 Jul 19;7(4)
mBio 2016 Jul 19;7(4)
Rheb promotes cancer cell survival through p27Kip1-dependent activation of autophagy.
Campos T, Ziehe J, Palma M, Escobar D, Tapia JC, Pincheira R, Castro AF
Molecular carcinogenesis 2016 Feb;55(2):220-9
Molecular carcinogenesis 2016 Feb;55(2):220-9
A dual role for autophagy in a murine model of lung cancer.
Rao S, Tortola L, Perlot T, Wirnsberger G, Novatchkova M, Nitsch R, Sykacek P, Frank L, Schramek D, Komnenovic V, Sigl V, Aumayr K, Schmauss G, Fellner N, Handschuh S, Glösmann M, Pasierbek P, Schlederer M, Resch GP, Ma Y, Yang H, Popper H, Kenner L, Kroemer G, Penninger JM
Nature communications 2014;5:3056
Nature communications 2014;5:3056
Phospholipase D2 mediates survival signaling through direct regulation of Akt in glioblastoma cells.
Bruntz RC, Taylor HE, Lindsley CW, Brown HA
The Journal of biological chemistry 2014 Jan 10;289(2):600-16
The Journal of biological chemistry 2014 Jan 10;289(2):600-16
Autophagy is critical for pancreatic tumor growth and progression in tumors with p53 alterations.
Yang A, Rajeshkumar NV, Wang X, Yabuuchi S, Alexander BM, Chu GC, Von Hoff DD, Maitra A, Kimmelman AC
Cancer discovery 2014 Aug;4(8):905-13
Cancer discovery 2014 Aug;4(8):905-13
mTORC1 inhibition delays growth of neurofibromatosis type 2 schwannoma.
Giovannini M, Bonne NX, Vitte J, Chareyre F, Tanaka K, Adams R, Fisher LM, Valeyrie-Allanore L, Wolkenstein P, Goutagny S, Kalamarides M
Neuro-oncology 2014 Apr;16(4):493-504
Neuro-oncology 2014 Apr;16(4):493-504
Augmenting autophagy to treat acute kidney injury during endotoxemia in mice.
Howell GM, Gomez H, Collage RD, Loughran P, Zhang X, Escobar DA, Billiar TR, Zuckerbraun BS, Rosengart MR
PloS one 2013;8(7):e69520
PloS one 2013;8(7):e69520
Pazopanib and sunitinib trigger autophagic and non-autophagic death of bladder tumour cells.
Santoni M, Amantini C, Morelli MB, Liberati S, Farfariello V, Nabissi M, Bonfili L, Eleuteri AM, Mozzicafreddo M, Burattini L, Berardi R, Cascinu S, Santoni G
British journal of cancer 2013 Aug 20;109(4):1040-50
British journal of cancer 2013 Aug 20;109(4):1040-50
Macroautophagy abnormality in essential tremor.
Kuo SH, Tang G, Ma K, Babij R, Cortes E, Vonsattel JP, Faust PL, Sulzer D, Louis ED
PloS one 2012;7(12):e53040
PloS one 2012;7(12):e53040
Autophagy induced by resveratrol prevents human prion protein-mediated neurotoxicity.
Jeong JK, Moon MH, Bae BC, Lee YJ, Seol JW, Kang HS, Kim JS, Kang SJ, Park SY
Neuroscience research 2012 Jun;73(2):99-105
Neuroscience research 2012 Jun;73(2):99-105
Involvement of macroautophagy in multiple system atrophy and protein aggregate formation in oligodendrocytes.
Schwarz L, Goldbaum O, Bergmann M, Probst-Cousin S, Richter-Landsberg C
Journal of molecular neuroscience : MN 2012 Jun;47(2):256-66
Journal of molecular neuroscience : MN 2012 Jun;47(2):256-66
Melatonin attenuates kainic acid-induced neurotoxicity in mouse hippocampus via inhibition of autophagy and α-synuclein aggregation.
Chang CF, Huang HJ, Lee HC, Hung KC, Wu RT, Lin AM
Journal of pineal research 2012 Apr;52(3):312-21
Journal of pineal research 2012 Apr;52(3):312-21
"Autophagic flux" in normal mouse tissues: focus on endogenous LC3A processing.
Zois CE, Giatromanolaki A, Sivridis E, Papaiakovou M, Kainulainen H, Koukourakis MI
Autophagy 2011 Nov;7(11):1371-8
Autophagy 2011 Nov;7(11):1371-8
Silica nanoconstruct cellular toleration threshold in vitro.
Herd HL, Malugin A, Ghandehari H
Journal of controlled release : official journal of the Controlled Release Society 2011 Jul 15;153(1):40-8
Journal of controlled release : official journal of the Controlled Release Society 2011 Jul 15;153(1):40-8
Pathogenic lysosomal depletion in Parkinson's disease.
Dehay B, Bové J, Rodríguez-Muela N, Perier C, Recasens A, Boya P, Vila M
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Sep 15;30(37):12535-44
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Sep 15;30(37):12535-44
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: LC3A Antibody [NBP1-19167] - Image shows a specific band for LC3 in 0.5 mg/mL of Neuro2A lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LC3/MAP1LC3A Antibody [NBP1-19167] - Human brain lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LC3A Antibody [NBP1-19167] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-LC3A in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the detection LC3 II upon chloroquine treatment.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: LC3A Antibody [NBP1-19167] - Analysis of a FFPE tissue section of mouse liver using LC3 antibody at 1:300 dilution. The signal was developed using HRP-labelled secondary antibody and DAB reagent, and the sections/nuclei were further counterstained with hematoxylin. Note the diffused cytoplasmic staining of LC3 in all of the hepatocytes and other liver cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: LC3A Antibody [NBP1-19167] - Analysis of a FFPE tissue section of mouse brain using LC3 antibody at 1:300 dilution. The signal was developed using HRP-labelled secondary antibody and DAB reagent, and the sections/nuclei were further counterstained with hematoxylin. Note the diffused cytoplasmic staining of LC3 in all of the cells with highest positivity in various neurons.
