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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PAB8066 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB8066, RRID:AB_1710138
- Product name
- MAP1LC3A (phospho S12) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of MAP1LC3A.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Degrade or die: a dual function for autophagy in the plant immune response.
Autophagy: dual roles in life and death?
Autophagy in metazoans: cell survival in the land of plenty.
Greenberg JT
Developmental cell 2005 Jun;8(6):799-801
Developmental cell 2005 Jun;8(6):799-801
Autophagy: dual roles in life and death?
Baehrecke EH
Nature reviews. Molecular cell biology 2005 Jun;6(6):505-10
Nature reviews. Molecular cell biology 2005 Jun;6(6):505-10
Autophagy in metazoans: cell survival in the land of plenty.
Lum JJ, DeBerardinis RJ, Thompson CB
Nature reviews. Molecular cell biology 2005 Jun;6(6):439-48
Nature reviews. Molecular cell biology 2005 Jun;6(6):439-48
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoblots of Phospho-MAP1LC3A in CHO cell culture. MAP1LC3 and MAP1LC3 S12A mutant vectors were transfected into CHO cells. The cell lysates were separated with SDS-PAGE and blotted with MAP1LC3A (phospho S12) polyclonal antibody (Cat # PAB8066). MAP1LC3 = microtubule-associated protein light chain-3; S12A = replacement of the amino acid position 12 serine of MAP1LC3 with alanine. WT = wildtype MAP1LC3-transfected cell lysates; S12A = MAP1LC3 S12A mutant-transfected cell lysates; Empty vector = vector with no MAP1LC3 gene. Molecular size: MAP1LC3-I = 16 KDa, and MAP1LC3-II = 14 KDa.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Dot blot analysis of MAP1LC3A (phospho S12) polyclonal antibody (Cat # PAB8066) and Nonphospho-MAP1LC3 antibody on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.
- Validation comment
- Dot Blot (Peptide)
