ABIN1449623
antibody from antibodies-online
Targeting: MAP1LC3A
ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3
Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [5]
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Validation data
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- Product number
- ABIN1449623 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (pSer12) antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
Submitted references Rab5 and class III phosphoinositide 3-kinase Vps34 are involved in hepatitis C virus NS4B-induced autophagy.
Degrade or die: a dual function for autophagy in the plant immune response.
Autophagy: dual roles in life and death?
Autophagy in metazoans: cell survival in the land of plenty.
Eating oneself and uninvited guests: autophagy-related pathways in cellular defense.
Autophagy in health and disease: a double-edged sword.
LC3 conjugation system in mammalian autophagy.
HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates.
Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B.
Su WC, Chao TC, Huang YL, Weng SC, Jeng KS, Lai MM
Journal of virology 2011 Oct;85(20):10561-71
Journal of virology 2011 Oct;85(20):10561-71
Degrade or die: a dual function for autophagy in the plant immune response.
Greenberg JT
Developmental cell 2005 Jun;8(6):799-801
Developmental cell 2005 Jun;8(6):799-801
Autophagy: dual roles in life and death?
Baehrecke EH
Nature reviews. Molecular cell biology 2005 Jun;6(6):505-10
Nature reviews. Molecular cell biology 2005 Jun;6(6):505-10
Autophagy in metazoans: cell survival in the land of plenty.
Lum JJ, DeBerardinis RJ, Thompson CB
Nature reviews. Molecular cell biology 2005 Jun;6(6):439-48
Nature reviews. Molecular cell biology 2005 Jun;6(6):439-48
Eating oneself and uninvited guests: autophagy-related pathways in cellular defense.
Levine B
Cell 2005 Jan 28;120(2):159-62
Cell 2005 Jan 28;120(2):159-62
Autophagy in health and disease: a double-edged sword.
Shintani T, Klionsky DJ
Science (New York, N.Y.) 2004 Nov 5;306(5698):990-5
Science (New York, N.Y.) 2004 Nov 5;306(5698):990-5
LC3 conjugation system in mammalian autophagy.
Tanida I, Ueno T, Kominami E
The international journal of biochemistry & cell biology 2004 Dec;36(12):2503-18
The international journal of biochemistry & cell biology 2004 Dec;36(12):2503-18
HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates.
Tanida I, Sou YS, Ezaki J, Minematsu-Ikeguchi N, Ueno T, Kominami E
The Journal of biological chemistry 2004 Aug 27;279(35):36268-76
The Journal of biological chemistry 2004 Aug 27;279(35):36268-76
Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B.
He H, Dang Y, Dai F, Guo Z, Wu J, She X, Pei Y, Chen Y, Ling W, Wu C, Zhao S, Liu JO, Yu L
The Journal of biological chemistry 2003 Aug 1;278(31):29278-87
The Journal of biological chemistry 2003 Aug 1;278(31):29278-87
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunoblots of SH-SY5Y cells treated with MPP+ for 24h was probed with AP32191PU-N. The data shows that treatment with MPP+ showed no significant change in level of LC3.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunoblots of SH-SY5Y cells treated with rapamycin for 1 h was probed with AP32191PU-N. The data shows that treatment with rapamycin showed no significant change in level of LC3.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Dot blot analysis of Phospho-LC3 (APG8a) - Ser12 Antibody (Cat.-No AP32191PU-N) and Non phospho-LC3 (APG8a) Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 μg/ml.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunoblots of phosphorylated LC3 (phospho-LC3) in CHO cell culture. LC3 and LC3 S12A mutant vectors were transfected into CHO cells. The cell lysates were separated with SDS-PAGE and blotted with anti-phospho-LC3 S12 antibody. LC3 = microtubule-associated protein light chain-3; S12A = replacement of the amino acid position 12 serine of LC3 with alanine. WT = wildtype LC3-transfected cell lysates; S12A = LC3 S12A mutant-transfected cell lysates; Empty vector = vector with no LC3 gene. Molecular size: LC3-I = 16kDa, and LC3-II = 14 kDa
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Something like SH-SY5Y cells expressing GFP-LC3-WT or-S12D treated with rapamycin or vehicle for 1h.