NB100-1774

antibody from Novus Biologicals

Targeting: SRSF2 PR264, SC-35, SC35, SFRS2, SFRS2A

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Immunoelectron microscopy (Data presented on provider website)

Antibody Data

Product number

NB100-1774

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-1774, RRID:AB_10128431

Product name

Mouse Monoclonal SC35 Antibody

Antibody type

Monoclonal

Description

Unpurified. Recognizes a phospho-epitope on the non-snRNP (small nuclear ribonucleoprotein particles) factor SC-35. The antibody reacts with the splicing factor SC-35 and with the SC-35-related non-snRNP factor SF2/ASF.

Reactivity

Human, Mouse, Rat, Drosophila

Host

Mouse

Antigen sequence

This antibody recognizes a phospho-
epitope of the non-snRNP (small nuc
lear ribonucleoprotein particles) f
actor SC35.


Isotype

IgG

Vial size

0.1 ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

The interferon stimulated gene 20 protein (ISG20) is an innate defense antiviral factor that discriminates self versus non-self translation.

Wu N, Nguyen XN, Wang L, Appourchaux R, Zhang C, Panthu B, Gruffat H, Journo C, Alais S, Qin J, Zhang N, Tartour K, Catez F, Mahieux R, Ohlmann T, Liu M, Du B, Cimarelli A
PLoS pathogens 2019 Oct;15(10):e1008093

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Matrin 3-dependent neurotoxicity is modified by nucleic acid binding and nucleocytoplasmic localization.

Malik AM, Miguez RA, Li X, Ho YS, Feldman EL, Barmada SJ
eLife 2018 Jul 17;7

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Reactive oxygen species exert opposite effects on Tyr23 phosphorylation of the nuclear and cortical pools of annexin A2.

Grindheim AK, Hollås H, Raddum AM, Saraste J, Vedeler A
Journal of cell science 2016 Jan 15;129(2):314-28

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Gadd45a is an RNA binding protein and is localized in nuclear speckles.

Sytnikova YA, Kubarenko AV, Schäfer A, Weber AN, Niehrs C
PloS one 2011 Jan 7;6(1):e14500

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A potential molecular mechanism for regulating pre-mRNA splicing of implantation-related genes through unique uterine expression of splicing factor SC35 in women and rhesus monkeys.

Nie GY, Hampton AL, Fu GQ, Liu YX, Findlay JK, Salamonsen LA
Reproduction (Cambridge, England) 2002 Aug;124(2):209-17

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A potential molecular mechanism for regulating pre-mRNA splicing of implantation-related genes through unique uterine expression of splicing factor SC35 in women and rhesus monkeys.

Nie GY, Hampton AL, Fu GQ, Liu YX, Findlay JK, Salamonsen LA
Reproduction (Cambridge, England) 2002 Aug;124(2):209-17

---

Associations between distinct pre-mRNA splicing components and the cell nucleus.

Spector DL, Fu XD, Maniatis T
The EMBO journal 1991 Nov;10(11):3467-81

---

Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus.

Fu XD, Maniatis T
Nature 1990 Feb 1;343(6257):437-41

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SC35 Antibody (SC-35) [NB100-1774] - SC-35 recombinant protein (3 ug) was separated on SDS-PAGE and probed with Mouse Anti-Splicing Factor SC-35 Clone: SC-35. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:100; 2. Antibody dilution 1:250; 3. Antibody dilution 1:500; 4. Antibody dilution 1:1,000