Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-35204 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATG7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with chicken, mouse and rat based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATG7 in 293 cell lysate (35 µg/lane) using an ATG7 polyclonal antibody (Product # PA5-35204).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATG7 in 293 cell lysate transiently transfected with the ATG7 gene (2 µg/lane) using an ATG7 polyclonal antibody (Product # PA5-35204).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATG7 in 293 cell lysate transiently transfected with the ATG7 gene (2 µg/lane) using an ATG7 polyclonal antibody (Product # PA5-35204).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ATG7 in U-251 cells using an ATG7 polyclonal antibody (Product # PA5-35204) followed by detection using a fluorescent conjugated secondary antibody (green) at a dilution of 1:400. Nuclei were stained with Dapi (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ATG7 in formalin-fixed, paraffin-embedded human hepatocarcinoma tissue using an ATG7 polyclonal antibody (Product # PA5-35204) followed by DAB staining.